Gold nanoparticles

A$70.00A$300.00

Gold nanoparticles (colloidal gold) of different sizes, citrate-capped.

Login to order

Description

Citrate-capped gold for passive adsorption in aqueous 2 mM sodium citrate. The concentration and surface chemistry of our gold nanoparticles are ideal to be used with the protocol developed by the Mirkin group (Hill et al., 2006) for the high-density conjugation to nucleic acids and aptamers (Chen et al., 2020).

 

The molar concentration of our gold nanoparticles is determined from TEM and UV-Vis spectra, as described by Hurst et al. (2006) and Liu et al. (2007). Exact specifications for each batch are reported in the Certificate of Analysis.

 

Product Data Sheet and Protocol

 

Safety Data Sheet

 

References

  • H. D. Hill, C. A. Mirkin. The bio-barcode assay for the detection of protein and nucleic acid targets using DTT-induced ligand exchange. Nature Protocols, 2006, 1, 324–336. (link)
  • X. Chen, F. Lisi, P. Bakthavathsalam, G. Longatte, S. Hoque, R. D. Tilley, J. J. Gooding. Impact of the Coverage of Aptamers on a Nanoparticle on the Binding Equilibrium and Kinetics between Aptamer and Protein. ACS sensors, 2020, 6, 2, 538–545. (link)
  • X. Liu, M. Atwater, J. Wang, Q. Huo. Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids and Surfaces B: Biointerfaces, 2007, 58, 1, 3-7. (link)
  • Sarah J. Hurst, Abigail K. R. Lytton-Jean, and Chad A. Mirkin. Maximizing DNA Loading on a Range of Gold Nanoparticle Sizes. Anal. Chem. 2006, 78, 24, 8313–8318. (link)

 

Additional information

Size

10 nm, 30 nm, 50 nm

Capping agent

Citrate-capped

Volume

15 mL

Concentration

OD = 0.2 (2 nM), OD = 1 (0.33 nM), OD = 4 (0.27 nM)